Product Code: MDS033
Product Description:Our Coronavirus SARS-CoV-2 RNA Extraction Kit / RNA Isolation Kit offers simple solutions for RNA isolation using Silica based Magnetic Beads. Our kit is designed to extract and purify RNA. Recovered RNA is compatible for COVID-19 RT-PCR Testing. We use highest quality magnetic beads and buffer solutions for pure RNA extraction in less than 20 mins. Compatible with Manual and Automated Platforms Maintain Purity and Integrity of Extracted RNA Compatible with all COVID-19 RTPCR Kits Extraction in high quantity of Total RNA Many Sample types supported including cells, blood,tissue and FFPE tissues
Intended UseViral RNA Extraction Kit (Magnetic Bead Method) is for the extraction, enrichment and purification of nucleic acid (RNA) in samples. Its processed products are for clinical in vitro diagnostics by medical professionals.
PrincipleThe magnetic Beads in the kit have specific polymeric groups of adsorbed nucleic acid (RNA) on the surface. In special conditions like hypersaline, cells or viruses in the samples lyse rapidly and release nucleic acids, which are specifically adsorbed by magnetic Beads. Nucleic acids on the magnetic beads will be separated from the liquid phase when the magnetic separator is used. Residual impurities and inhibitors in the liquid phase are removed by washing with wash buffer. In our kit, there are two washing steps to ensure high yield of RNA. Finally, nucleic acids are eluded from the magnetic beads by changing the liquid phase conditions, so as to separate nucleic acid rapidly and efficiently. The extracted viral RNA can be directly used for the downstream applications such as PCR, RT-PCR, qPCR, qRT-PCR etc.
Shipping and storageCarrier RNA in solubilized form has to be stored at -20°C for one year (Shipped in dry ice). All solutions should be stored at room temperature unless otherwise stated. Under these conditions, all buffers and reagents can be stored until the expiration date on the kit box, without showing any reduction in performance.
|Kit Components||100 Reactions||500 Reactions|
|Micro Centrifugal Tubes||100||Zip Pouch||500||Zip Pouch|
|Lysis cum binding buffer||50 mL||1 x 50 mL||250 mL||5 x 50 mL|
|Magnetic beads||5 mL||1 x 50 mL||25 mL||1 x 50 mL|
|Buffer 1||50 mL||1 x 50 mL||250 mL||5 x 50 mL|
|*Wash Buffer||50 mL||1 x 50 mL||250 mL||5 x 50 mL|
|Elution Buffer||6 mL||1 x 50 mL||30 mL||1 x 50 mL|
|Carrier RNA Solubilized Form (Supplied Separately)||1.0 mL||1 x 1.5 mL||5.0 mL||1 x 15 mL|
*Wash Buffer is supplied as a concentrate (10 mL in 50 mL Bottle), Before using for the ﬁrst time, add 40 mL of ethanol (96-100%) to each bottle. Wash Buffer is stable for 1 year when stored closed at room temperature, but only until the kit expiration date.